IL2 Mouse Monoclonal Antibody [Clone ID: 1H10-1-1]

Specifications

Product Data
Clone Name	1H10-1-1
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-2 when used with Biotin conjugated anti-Human IL-2 (Cat.-No PP1024B) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml. Western Blot: To detect human IL-2 by Western blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: 2.5-5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium citrate buffer is recommended. Positive Control: Human Breast invasive ductal carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified (>98%) E.coli derived recombinant Human IL-2 (Cat.-No PA075)
Specificity	Detects Human Interleukin 2. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution the antibody can be stored at 2-8°C for two weeks or (in aliquots) at -20°C for at least 6 months. Avoid repeated freezing and thawing.
Gene Name	interleukin 2
Database Link	Entrez Gene 3558 Human UniProt ID P60568
Background	Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
Synonyms	IL-2, TCGF
Reference Data

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