HGF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA (1 - 15 µg/ml). Western blot (1 - 2 µg/ml).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant human HGF
Specificity	This antibody detects HGF.
Formulation	PBS, pH 7.4 State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore in sterile water/PBS to a concentration of > 0.5 mg/ml.
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at 2 - 8°C for one month and at -20°C for one year from despatch. Avoid repeated freezing and thawing. Following reconstitution the product is stable for one month at 2 - 8°C.
Gene Name	hepatocyte growth factor
Database Link	Entrez Gene 3082 Human UniProt ID P14210
Background	Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitious protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmatic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.
Synonyms	Scatter factor, Hepatopoeitin-A, HPTA
Reference Data

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