VEGFA (Isoform 165) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IP, WB
Recommend Dilution	Neutralization: 1-2.5 µg/ml, to yield one-half maximal inhibition (ND50) of the biological activity of Human VEGF (50 ng/ml). ELISA: 0.5 µg/ml, allows the detection of 0.5-1.0 ng/well of recombinant Human VEGF165 or other VEGF splice forms. Western Blot: 1-5 μg/ml. Immunoprecipitation: 1-5 µg/ml lysate or reaction volume.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified Recombinant Human VEGF165 [Ala27-Arg191] produced in Insect cells (Cat.-No DA3514X)
Specificity	The antibody will detect Human VEGF-A. Other species not tested.
Formulation	PBS, pH 7.4 containing no preservatives State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor A
Database Link	Entrez Gene 7422 Human UniProt ID P15692
Background	Human Vascular Endothelial Growth Factor VEGF165, a 23kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Reference Data

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