Factor XIIIa (F13A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen and Paraffin Sections: 1/25-1/50 dilution in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Placenta.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein corresponding to A-subunit of coagulation Factor XIII a
Specificity	This antibody reacts with a 160 kDa (unrecuced dimer) and 80 kDa (reduced monomer) protein. Cellular Localization: Cytoplasmic and nuclear.
Formulation	State: Purified State: Liquid purified IgG fraction Preservative: Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	160 kDa (unreduced dimer) and 80kDa (reduced monomer)
Gene Name	coagulation factor XIII A chain
Database Link	Entrez Gene 2162 Human UniProt ID P00488
Background	Factor XIII in both reduced and nonreduced forms. It does not react with human Factor XIII B-chain or human Factor XII. Factor XIII is a β-globulin found in plasma and is composed of two subunits. Factor XIII-A is the catalytic subunit and is a dimmer of M.W. 160kDa. Factor XIII is present in plasma as an α2β2 heterodimer (M.W. 320kDa); whereas in platelets, only the α2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.
Synonyms	Coagulation factor XIII A chain, F13A, Transglutaminase A chain
Reference Data

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