VEGF Receptor 2 (KDR) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Sections: Use a 1/50-1/100 dilution in an ABC method (incubation time: 30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended positive control: Angiosarcoma.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide derived from the C-terminus of the precursor form of the Mouse Flk-1
Specificity	This antibody reacts with a 180 kD protein, known as Flk-1/KDR/Vascular endothelial growth factor receptor 2. Cellular Localization: Cytoplasmic.
Formulation	State: Purified State: Liquid purified IgG fraction Preservative: < 0.1% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	kinase insert domain receptor
Database Link	Entrez Gene 3791 Human UniProt ID P35968
Background	CD309 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Note	Mol. Wt. of Antigen: ~180 kDa
Reference Data

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