VEGFC Mouse Monoclonal Antibody [Clone ID: 9E7]

Specifications

Product Data
Clone Name	9E7
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1-5 µg/ml. Immunofluorescence: 2-10 µg/ml. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human VEGF-C protein (Cat.-No AR01001PU-N).
Specificity	This antibody detects VEGF-C in Western Blots. No cross-reactivity is shown with VEGF-A.
Formulation	Phosphate-Buffered Saline (PBS) State: Purified State: Lyophilized (0.2µm filtered) purified IgG fraction
Reconstitution Method	Restore in Sterile Water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor C
Database Link	Entrez Gene 114111 RatEntrez Gene 7424 Human UniProt ID P49767
Background	VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Synonyms	VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma

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