VEGF Receptor 2 (KDR) Mouse Monoclonal Antibody [Clone ID: 4H3]

Specifications

Product Data
Clone Name	4H3
Applications	ELISA, FC, WB
Recommend Dilution	ELISA: 1-10 µg/ml. Western Blot: 2-5 µg/ml. Flow Cytometry analysis and cell sorting: 2-10 µg/ml. Immunofluorescence/ Immunohistochemistry: 6-30 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular KDR (D7) (110 kDa) protein (Cat.-No AR26018PU-N)
Specificity	This unconjugated antibody will detect native Human VEGFR-2/KDR in ELISA experiments and on the surface of different Human cell types.
Formulation	PBS Label: Biotin State: Lyophilized purified Ig fraction Stabilizer: BSA (50X) Preservative: 0.02% Sodium Azide
Reconstitution Method	Restore with sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Protein G Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	kinase insert domain receptor
Database Link	Entrez Gene 3791 Human UniProt ID P35968
Background	VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Reference Data

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