TIE2 (TEK) Mouse Monoclonal Antibody [Clone ID: Cl.2]

Specifications

Product Data
Clone Name	Cl.2
Applications	ELISA, FC, WB
Recommend Dilution	ELISA: 1-2 µg/ml. Western Blot. FACS analysis and Cell sorting: 2-5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular domain of TIE-2
Specificity	The unconjugated Monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different Human cell types.
Formulation	0.1M Tris-Cl, 0.2M NaCl, pH 7.4 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 50x BSA Preservative: 0.02% Sodium Azide
Reconstitution Method	Restore to a concentration of 50 µg/ml with sterile PBS solution containing 0.1% BSA.
Purification	Protein G Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C for one month or at -20°C for 6 months. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	TEK receptor tyrosine kinase
Database Link	Entrez Gene 7010 Human UniProt ID Q02763
Background	TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.
Synonyms	TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK
Reference Data

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