VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: FLTEWF]

Specifications

Product Data
Clone Name	FLTEWF
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA: 1-10 µg/ml. Western blotting: 1-10 µg/ml. Immunoprecipitation: 1-5 µg/ml lysate or reaction volume.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539)
Specificity	The monoclonal antibody will recognize recombinant and naturally occurring form of Human VEGFR-1/Flt-1.
Formulation	PBS pH 7.4 containing no preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 1
Database Link	Entrez Gene 2321 Human UniProt ID P17948
Background	Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Synonyms	VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
Reference Data

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