Cyclin E1 (CCNE1) Mouse Monoclonal Antibody [Clone ID: HE12]

Specifications

Product Data
Clone Name	HE12
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to human cyclin E protein.
Specificity	This antibody recognizes a doublet of 50 kD and a single band of 42 kD identified as Cyclin E. Cellular Localization: Nuclear
Formulation	State: Supernatant State: Liquid purified Ig fraction containing Sodium Azide as preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin E1
Database Link	Entrez Gene 898 Human UniProt ID P24864
Background	Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16. During tumor cell and normal cell proliferation, cyclins activate the cyclin-dependent protein kinases, which in turn controls progression through the G1 phase of the cell cycle.
Synonyms	Cyclin-E1, CCNE1, CCNE
Reference Data

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