Androgen Receptor (AR) Mouse Monoclonal Antibody [Clone ID: AR441]

Specifications

Product Data
Clone Name	AR441
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/50-1/100. Immunohistochemistry on Paraffin Embedded Sections: Use at 1/100-1/200 with Polymer Detection system.  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 or 1 mM EDTA, pH 8.0 buffer prior to immunostaining.  Incubation Time: 30 minutes at RT.  Recommended Positive Control: Prostate carcinoma.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were injected with a synthetic peptide, amino acid 340-356 of Human Androgen Receptor
Specificity	This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors. Cellular Localization: Nuclear.
Formulation	State: Purified State: Liquid purified Ig fraction Preservative: Less than 0.1% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	110 kDa
Gene Name	androgen receptor
Database Link	Entrez Gene 367 Human UniProt ID P10275
Background	The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.
Synonyms	Dihydrotestosterone receptor, DHTR, NR3C4
Reference Data

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