CD66a/b/c/e Mouse Monoclonal Antibody [Clone ID: TET2]

Specifications

Product Data
Clone Name	TET2
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1.0 µg/106 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western Blot: 4 µg/ml. Immunohistology: 1-2 µg/106 cells (on Cryosections).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	TET2 was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
Specificity	This antibody recognizes CD66a/b/c/e.
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immuno-globulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
Synonyms	CEACAM1, CEACAM5, CEACAM6, CEACAM8, BGP, CD66a, CD66b, CD66c, CD66d
Note	Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
Reference Data

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