CD66c/e Mouse Monoclonal Antibody [Clone ID: MUS]

Specifications

Product Data
Clone Name	MUS
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1.2 µg/106 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western blot: 4 µg/ml. Immunohistochemistry: 1-2 µg/106 cells (on Cryosections).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	MUS was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
Specificity	This antibody reacts to CD66c/e.
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly upregulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
Synonyms	CEACAM5, Carcinoembryonic antigen, CEA, Meconium Antigen 100, Normal Cross-reacting Antigen, CEACAM6, NCA
Reference Data

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