CDK1 Mouse Monoclonal Antibody [Clone ID: POH-1]

Specifications

Product Data
Clone Name	POH-1
Applications	IF, IHC, IP, WB
Recommend Dilution	Western Blot: 1-2 µg/ml. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml.  Positive Control: Thymus.
Reactivity	Human, Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed full-length Human Cdk1 protein.
Specificity	The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.  Negative Species: Mouse, Rat, Xenopus and Drosophila.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase 1
Database Link	Entrez Gene 983 Human UniProt ID P06493
Background	The p34cdc2 is a 34 kDa phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the cell cycle. Expression of p34cdc2 reflects the proliferating potential of positively staining tissue. The maximal activity of p34cdc2 is achieved when it is fully dephosphorylated. It is a 34kD catalytic subunit of maturation promoting factor (MPF) and complexes with both cyclin A and cyclin B in mammalian cells.
Synonyms	CDK1, CDC2, CDC28A, CDKN1, P34CDC2, p34 protein kinase
Reference Data

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