TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: 8C4]

Specifications

Product Data
Clone Name	8C4
Applications	ELISA, FN, IHC
Recommend Dilution	ELISA. Neutralizing Activity: This MAb neutralizes TGF beta activity in vitro in inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-beta in the NRK-49F colony forming assays. The effect of micro-injection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this MAb was able to neutralize effectively the bioactivity of TG beta in vivo. Immunohistochemistry: May be used in immunohistochemical techniques to locate TGF-beta within tissues. Western Blotting：This antibody can detect 40ng/lane of human TGF-beta on Western Blot. The blot below was stained with HRP substrate 4-Chloro-1-naphthol and H2O2.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	TGF-beta from Human platelets.
Specificity	Western blotting demonstrated that this TGF-beta antibody reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-beta under non-reducing and reducing condition respectively. Recognizes both Human platelet-derived and recombinant TGF beta in ELISA.
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Azide Free State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.00 mg/ml.
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	transforming growth factor beta 1
Database Link	Entrez Gene 7040 Human UniProt ID P01137
Background	TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein.
Synonyms	TGFB, Transforming growth factor beta-1, TGF-beta-1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors
Protein Pathways	Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway

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