TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: TB21]

Specifications

Product Data
Clone Name	TB21
Applications	ELISA, FN, IHC, WB
Recommend Dilution	ELISA: This antibody recognizes both Human platelet-derived and recombinant TGF-beta in ELISA. Functional Assay: This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of micro-injection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry on FFPE and Frozen Sections: This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and Human breast carcinoma at a dilution of 1/1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. In another Western Blot experiment the antibody used at 5-20 ng/ml, detected TGF-β at 200 ng/lane.
Reactivity	Human, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	TGF-beta from Human platelets.
Specificity	This antibody detects Transforming Growth Factor beta/TGF beta. Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-β under both non-reducing and reducing conditions respectively. This antibody recognizes both human platelet-derived and recombinant TGF-β in ELISA.
Formulation	0.01M PBS, pH 7.0 without preservatives State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 0.5 ml double distilled water.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	transforming growth factor beta 1
Database Link	Entrez Gene 7040 Human UniProt ID P01137
Background	TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein.
Synonyms	TGFB, Transforming growth factor beta-1, TGF-beta-1
Reference Data

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