TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: 2C6]

Specifications

Product Data
Clone Name	2C6
Applications	ELISA, FN, IHC, WB
Recommend Dilution	ELISA. Western Blot: MAb 2C6 at a concentration of 50-500 ng/mL will allow visualization of 100-500 ng/lane of TGF-β. Neutralization: This MAb neutralizes TGF-β activity in vitro in inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-β in the NRK-49F colony forming assays. The effect of micro-injection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this MAb was able to neutralize effectively the bioactivity of TGF-β in vivo. Immunohistochemistry: May be used in immunohistochemical techniques to locate TGF-β within tissues.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	TGF-β from human platelets
Specificity	Western blotting demonstrated that this MAb reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-β under non-reducing and reducing condition respectively. This MAb recognizes both human platelet-derived and recombinant TGF-β in enzyme-linked immunosorbent assay (ELISA).
Formulation	0.01M PBS, pH 7.0 State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Protein G affinity purified
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	transforming growth factor beta 1
Database Link	Entrez Gene 7040 Human UniProt ID P01137
Background	TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein.
Synonyms	TGFB, Transforming growth factor beta-1, TGF-beta-1
Reference Data

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