RAF1 Mouse Monoclonal Antibody [Clone ID: 410]

Specifications

Product Data
Clone Name	410
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot: An antibody concentration of 1 µg/ml will allow visualization of 0.2 µg/lane of Human Raf-1. A main band (75 kDa) was visible.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Raf-1 (mw:75 kDa)
Specificity	This antibody reacts to Raf-1 N-terminal (1-240 a.a.)
Formulation	0.01M PBS, pH 7.2 without preservatives State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/mL
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Raf-1 proto-oncogene, serine/threonine kinase
Database Link	Entrez Gene 5894 Human UniProt ID P04049
Background	The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
Synonyms	C-RAF, Raf-1, cRaf
Note	Not suitable for the detection of Raf-1 expression and phosphorylation in Hela cells.
Reference Data

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