Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]

CAT#: DM080P

Neurofilament (NEFL) (+ Neurofilament H) mouse monoclonal antibody, clone 2F11, Purified



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CNY 5,290.00


货期*
5周

规格
    • 100 ug

Cited in 2 publications.

Product images

Specifications

Product Data
Clone Name 2F11
Applications IHC, WB
Recommend Dilution Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Embedded Sections.
Recommended Dilutions: 1/25–1/200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting.
Reactivity Feline, Human, Mouse, Opossum, Rabbit, Rat
Host Mouse
Clonality Monoclonal
Immunogen Human neurofilament preparation
Specificity 2F11 reacts exclusively with the phosphorylated isoform of the 70 kD neurofilament protein.
This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.
Formulation PBS
State: Purified
State: Liquid purified antibody
Preservative: 0.09% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size 68 kDa and 200 kDa (phosphorylated forms of these subunits).
Gene Name neurofilament, light polypeptide
Background Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation
Synonyms NF-L,NEFL, NF68, 68 kDa neurofilament protein
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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