Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]
CAT#: DM080P
Neurofilament (NEFL) (+ Neurofilament H) mouse monoclonal antibody, clone 2F11, Purified
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CNY 5,290.00
货期*
5周
规格
Cited in 2 publications. |
Product images
Specifications
Product Data | |
Clone Name | 2F11 |
Applications | IHC, WB |
Recommend Dilution | Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections. Recommended Dilutions: 1/25–1/200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting. |
Reactivity | Feline, Human, Mouse, Opossum, Rabbit, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Human neurofilament preparation |
Specificity | 2F11 reacts exclusively with the phosphorylated isoform of the 70 kD neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. |
Formulation | PBS State: Purified State: Liquid purified antibody Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Predicted Protein Size | 68 kDa and 200 kDa (phosphorylated forms of these subunits). |
Gene Name | neurofilament, light polypeptide |
Database Link | |
Background | Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation |
Synonyms | NF-L,NEFL, NF68, 68 kDa neurofilament protein |
Reference Data |
Citations (2)
The use of this Antibodies has been cited in the following citations: |
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Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
,null,
Biomedicines
,PubMed ID 34829732
[NEFL]
|
Integrated genotype–phenotype analysis of long‐term epilepsy‐associated ganglioglioma, et al. Integrated genotype–phenotype analysis of long‐term epilepsy‐associated ganglioglioma
,null,
Brain Pathology
,PubMed ID 34355449
[NEFL]
|
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