Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: OVTL-12/30]

Specifications

Product Data
Clone Name	OVTL-12/30
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunocytochemistry. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100 Retrieval conditions: Pretreatment of tissue with proteolytic enzymes should be performed prior to staining. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Ovarian carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	OTN II ovarian carcinoma cell line.
Specificity	Antibody to Cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). OVTL 12-30 decorates specific subtypes of adenocarcinoma. Cellular Localization: cytoplasmic.
Formulation	State: Supernatant State: Liquid purified IgG fraction with 0.2% BSA and 15mM Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 7
Database Link	Entrez Gene 54474 HumanEntrez Gene 3855 Human UniProt ID P08729
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Keratin K7 is found in the columnar and glandular epithelium of the lung, cervix, breast, bile ducts, larger collecting ducts of the kidney and in mesothelium, but is normally absent from gastrointestinal epithelium, hepatocytes and myoepithelium. Several types of adenocarcinomas of the urinary tract are negative, while adenocarcinomas of the mammary gland, ovary and lung are positive. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression.
Synonyms	KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin
Reference Data

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