Carboxypeptidase M (CPM) Mouse Monoclonal Antibody [Clone ID: M27]

Specifications

Product Data
Clone Name	M27
Applications	FC, IHC, IP
Recommend Dilution	DDX0520P-50 / DDX0520P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Immunoprecipitation. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	pre-ALP cells (human B-lineage acute lymphoblastic leukaemia cell line)
Specificity	Human Carboxypeptidase M.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	carboxypeptidase M
Database Link	Entrez Gene 1368 Human UniProt ID P14384
Background	Monoclonal antibody (MoAb) M27 was generated after immunization of mice with the human B-lineage acute lymphoblastic leukemia cell line Pre-ALP. Under reducing conditions, MoAb M27 precipitated a 60- kD surface-membrane molecule from Pre-ALP cells. Expression cloning of Pre-ALP cDNA showed that M27 recognizes carboxypeptidase M (CPM), a cell-surface, zinc-dependent protease known to cleave off basic C-terminal amino acids from peptide hormones. Using M27 antibody, CPM was detected only at discrete B lymphocyte developmental stages, namely on committed precursors and on germinal center cells. CPM was also expressed on mature T cells, mainly after activation. These results provide the first description of a carboxy-peptidase on lymphoid cells. In addition, CPM was found on granulocytes and monocytes but not on their progenitors. CPM was found to be expressed with CD10 and CD13 respectively on lymphoid and myeloid cells. (de Saint Vis B et al., Blood 1995,86,1098-105).
Synonyms	CPM
Reference Data

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