MMP12 Mouse Monoclonal Antibody [Clone ID: 701E4.03]

Specifications

Product Data
Clone Name	701E4.03
Applications	ELISA, IP, WB
Recommend Dilution	DDX0284P-50 DDX0284P-100 Purified: Capture, Immunoprecipitation, Western Blot. Usage recommendation: *This monoclonal antibody may be used: Capture: 3μg/ml in Carbonate buffer (pH 9,6). Detection: 5μg/ml in PBS-BSA-tween. Positive standard: 1/200 = 10 ng/ml. *Optimal dilution should be determined by each laboratory for each application.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant HME.
Specificity	Human HME-MMP12. *For DDX0284 recognition of the 54kDa form, a pre-treatment of the samples with DTT is required (Demedts IK et al, 2006; Thorax, 61:196-201).
Formulation	Tris-NaCl pH 8 State: Purified
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	matrix metallopeptidase 12
Database Link	Entrez Gene 4321 Human UniProt ID P39900
Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is reported only in a few cells, including tissue macrophages and hypertrophic chondrocytes. MMP-12 is critical for invasion and destruction in pathologies such as aneurysm and emphysema. The predicted molecular mass of the HME proenzyme is 54 kDa. HME mRNA and protein were detected in human alveolar macrophages. Similar to murine macrophage metalloelastase, HME readily undergoes NH2- and COOH-terminal processing to a mature 22 kDa form. Both recombinant expressed in Escherichia Coli and native HME derived from human alveolar macrophageconditioned media degraded insoluble elastin. HME is a unique human metalloproteinase that displays elastolytic activity and is expressed in alveolar macrophages; MMP-12 mediates smoke-induced inflammation by releasing TNFα from macrophages, with subsequent endothelial activation, neutrophil influx, and proteolytic matrix breakdown caused by neutrophil-derived proteases. (Demedts IK et al, 2006; Thorax, 61:196-201).
Synonyms	Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophage elastase
Reference Data

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