DC SIGN (CD209) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 118A8.05]

Specifications

Product Data
Clone Name	118A8.05
Applications	FC, IHC, WB
Recommend Dilution	Antigen uptake, FACS surface, ImmunoHistoChemistry frozen sections, Western Blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	human (GMCSF + TNF) DC subset
Specificity	human (epitope in extracellular domain).
Formulation	Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles.
Gene Name	CD209 molecule
Database Link	Entrez Gene 30835 Human UniProt ID Q9NNX6
Background	We have generated an antibody recognizing an antigen which has a tissular distribution and a size (western blot) identical to DC-SIGN. This antibody does not recognize DC-SIGN 1 expressed in HeLa cells. DCSIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 is a type II membrane protein with an external mannose-binding C-type lectin domain, DC-SIGN is expressed by immature and mature dendritic cells (DC). In the skin, DC-SIGN+ DC are exclusively located in the dermis. DC-SIGN binds to ICAM-3 on resting T cells, establishing DC-T cell contact and adaptive immunity. DC-SIGN is a high affinity receptor for HIV gp120, allowing HIV capture and transmission to CD4+ T cells. In addition to HIV, DC-SIGN is a receptor for a number of other viral and cellular pathogens including Mycobacterium Tuberculosis, and is a major player in microbial evasion of the immune system. (Geijtenbeek, T et al, Cell; 2000; 100: 587-597; van Kooyk Y et al, Nat. Rev. Immunol.; 2003; 3: 697-709).
Synonyms	DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data

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