CD40L (CD40LG) Mouse Monoclonal Antibody [Clone ID: LL48]

Specifications

Product Data
Clone Name	LL48
Applications	FC, FN, IF
Recommend Dilution	Cell culture inhibition in MELR H3, Thymidine uptake proliferative assay Immunofluorescence. Flow cytometry, neutralization of CD40L biological effects. *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Chimeric CD40L-Ig-transfected COS-7 cells.
Specificity	Human CD40L.
Formulation	100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD40 ligand
Database Link	Entrez Gene 959 Human UniProt ID P29965
Background	CD40 ligand also known as TRAP, gp39, TNFSF5, is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF superfamily. CD40 ligand is expressed predominantly on activated CD4+ T cells and is also found on other cell types, including NK cells, mast cells, basophils and eosinophils. Genetic alterations in the CD40L results in the absence of a functional molecule on activated T cells and are responsible for the occurrence of X-linked hyper-IgM syndrome. LL48 clone is a CD40L neutralizing antibody and plays an important role in costimulatory interactions between T and B lymphocytes, and antigen-presenting cells and lymphocytes regulating the immune system at multiple levels. (Tong AW et al, 2001, Clin. Canc. Res., 7:691-703; Hill A. et al, 1993; Nature, 361:494-503; Péguet- Navarro J. et al, 1995; J. Immunol., 155:4241-4247).
Synonyms	CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.