CD14 Mouse Monoclonal Antibody [Clone ID: MOP9.25]

Specifications

Product Data
Clone Name	MOP9.25
Applications	FC, IHC
Recommend Dilution	Flow cytometry, cell depletion, IHC.  Usage recommendation:  *This monoclonal antibody may be used between 1-5 μg/ml for flow cytometry and 5-25 μg/ml for IHC
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	peripheral blood monocytes (patient with rheumatoid arthritis)
Specificity	human CD14
Formulation	State: Purified State: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 recognizes a human monocyte/macrophage antigen of 55 kDa. It reacts with blood monocytes and has a weak reactivity on blood granulocytes, and interfollicular macrophages. Different cytokines have an effect on CD14 expression: CD14 expression was studied in dendritic cells generation in vitro. After 5 days in GMCSF + IL4, CD14 disappears while CD1a expression appears. TNF-α skews monocyte differentiation form macrophages to dendritic cells. The antibody MOP9.25 is used for monocyte depletion before dendritic cells sorting. (Dimitriu-Bona A., et al, J. Immunol., 1983 ; 130: 145-152 ; Banchereau J. et al, Annu. Rev. Immunol., 2000; 18: 767-811; Chomarat P. et al, J. Immunol., 2003; 171: 2262-2269).
Synonyms	CD14
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.