Birch Pollen (allergen Bet v1) Mouse Monoclonal Antibody [Clone ID: 102H10.05]

Specifications

Product Data
Clone Name	102H10.05
Applications	Neutralize
Recommend Dilution	Binding to nitrocellulose-blotted Bet v 1, inhibition of human IgE binding to Bet v1, cross-competition with anti-Bet v1 mAbs. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use
Reactivity	Pollen
Host	Mouse
Clonality	Monoclonal
Immunogen	pollen grains from Betula alba
Specificity	Birch pollen Bet v 1 Cross-reactivity: Bet v 1 homologous plant allergens.
Formulation	State: Purified State: 100 μg in 200 μl Tris-NaCl pH 8
Concentration	lot specific
Purification	QMA Hyper-D Ion-exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	Bet v 1 and homologous proteins represent major allergens for almost 95% of patients allergic to tree pollen and approximately 70% of those allergic to fruits and vegetables. Birch pollen allergy is a frequent pathology with IgE reactivity against Bet v 1. A panel of 85 mouse monoclonal anti-Bet v 1 antibodies was raised as a tool to study the interaction of human IgE antibodies with Bet v 1. Cross-reactivities with Bet v 1 homologous plant allergens, modulation of IgE binding to Bet v 1, Bet v 1 immunopurification, and inhibition of histamine release are described in the original article. (Lebecque S et l, J Allergy Clin Immunol, 1997; 99:374-84).
Reference Data

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