Ptprc Mouse Monoclonal Antibody [Clone ID: OX-30]

Specifications

Product Data
Clone Name	OX-30
Applications	FC
Recommend Dilution	Suitable for use in Flow cytometry (See Protocol).
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Lymph node glycoproteins and cells.
Specificity	This antibody recognizes a monomorphic determinant of the rat leukocyte common antigen (CD45) (1). The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 kDa on thymocytes but molecular weight 170,000-220,000 kDa on other leukocytes.
Formulation	PBS with 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: PE State: Liquid purified IgG fraction. Label: Conjugated to
Concentration	lot specific
Purification	Protein G Chromatography.
Conjugation	PE
Storage Condition	Store the antibody at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 24699 Rat UniProt ID P04157
Background	The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95% of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytes. (2,3).
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, 0.5 µg-1.0 µg of CL111R or CL111RX per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: (Figure 1) Rat Strain: Wistar Cell Concentration: 1x10e6 cells per test. Antibody Concentration Used: 0.5 µg/10e6 cells. Isotypic Control: PE Mouse IgG2a. Cell-Source Percentage of cells stained above control: Thymus: 99.8% Spleen: 97.1% Lymph Node: 98.9% Strain Distribution: Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344 Positive: Wistar, Buffalo, Brown Norway, Fischer 344 Negative: none
Reference Data

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