Kit Rat Monoclonal Antibody [Clone ID: ACK4]

CAT#: CL042B

Kit rat monoclonal antibody, clone ACK4, Biotin



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CNY 3,619.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name ACK4
Applications FC
Recommend Dilution Flow Cytometry.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen IL-3 dependent mast cells derived from WB- +/+ mice
Donor: Wistar spleen
Fusion Partner: X63.653. Ag8
Specificity Anti-mouse CD117 monoclonal antibody recognizes the receptor tyrosine kinase, c-kit. The ligand for this receptor is steel factor (stem cell factor), which exists in both soluble and membrane form. The interaction between steel factor and c-kit is essential for the development of hematopoietic, gonadal and pigment stem cells. c-kit positive cells are a subset of CD34+ hematopoietic precursor cells and it is expressed on 5-10% of total adult bone marrow cells.
Formulation PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: Biotin
State: Liquid purified Ig fraction
Concentration lot specific
Purification Affinity chromatography on Protein G
Conjugation Biotin
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name kit oncogene
Synonyms SCFR, KIT
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M (cell separation medium).
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.2-0.1 mg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 μl of secondary antibody (Streptavidin-PE) at a 1/250 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 μl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: C3H/He
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.2 μg/10e6 cells
Isotypic Control: Biotin Rat IgG2a
Strain Distribution by Flow Cytometry Analysis:
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.2 mg /10e6 cells
Strains Tested: AKR, BALB/c, C3H/He,
Positive: AKR, BALB/c, C3H/He,
Negative: none
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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