Thy1 Mouse Monoclonal Antibody [Clone ID: 5a-8]

Specifications

Product Data
Clone Name	5a-8
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
Specificity	Mouse CD90. This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: FITC State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Protein G Chromatography.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
Synonyms	Thy-1, THY1, CDw90
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1/50 -1/100 dilution in 50 µl of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protected from light since most fluorochromes are light sensitive) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 1/50 in 50 µl/10e6 cells Isotypic Control: FITC Mouse IgG2b Cell Source Percentage of cells stained above control: Thymus: 97.4% Spleen: 52.6% Results - Strain Distribution: Tissue: Spleen Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 1/100 in 50 µl/10e6 cells Strains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/J Positive: C57BL/6, C3H/He, CBA/J, BALB/c, ATL Negative: AKR
Reference Data

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