CD11a / ITGAL Mouse Monoclonal Antibody [Clone ID: 8-6.2]

Specifications

Product Data
Clone Name	8-6.2
Applications	FC
Recommend Dilution	Flow Cytometry (See Protocols).
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	B6-Ly-1a Thymus, spleen and lymph node. Donor: 129/ReJ spleen Fusion Partner: P3-NS-1 Ag-4
Specificity	This CD11a Monoclonal Antibody identifies a cell surface glycoprotein consisting of two non-covalently associated chains with molecular weights of 180kDa (Alpha chain) (1) present on most common lymphocytes and T and B cells.
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: FITC State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Database Link	UniProt ID P24063
Background	CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.
Synonyms	Integrin alpha-L, LFA1, LFA-1
Note	Protocol: Flow Cytometry Analysis: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add ~1.0 µg* of CL013F or CL013FX. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: CBA/J Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 1.0 µg/106 cells Isotypic Control: FITC Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 98.5% Spleen: 92.2 Lymph Node: 96.8 Strain Distibution: Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 1.0 µg/10e6 cells Strains Tested: /6, BALB/c, AKR, CBA/J, C3H/HE Positive: C57BL/6, CBA/J, C3H/He Negative: BALB/c, AKR
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.