Cd4 Rat Monoclonal Antibody [Clone ID: CT-CD4]

Specifications

Product Data
Clone Name	CT-CD4
Applications	FC
Recommend Dilution	Flow Cytometry (see Protocols). (Reported to be useful in immunohistochemistry on acetone fixed frozen sections).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This CT-CD4 monolconal antibody (mAb) recognizes mouse CD4 (L3T4).
Formulation	PBS containing 0.09% Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD4 antigen
Database Link	Entrez Gene 12504 Mouse UniProt ID P06332
Background	CD4 (L3T4) is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC’s and in signal transduction via protein tyrosine kinase lck1.
Synonyms	T-cell surface antigen T4/Leu-3
Note	Protocol: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add ~1.0-0.5 µg of this Ab per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of (Streptavidin-PE) at a 1:20 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c Cell Concentration: 1x106 cells per test Antibody Concentration Used: 0.5 µg/106 cells Isotypic Control: Biotin Rat IgG2a
Reference Data

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