HB EGF (HBEGF) Mouse Monoclonal Antibody [Clone ID: OTI8B5]

Specifications

Product Data
Clone Name	OTI8B5
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 20-208 of human HBEGF (NP_001936) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	22.9 kDa
Gene Name	heparin binding EGF like growth factor
Database Link	NP_001936 Entrez Gene 1839 Human UniProt ID Q99075
Background	Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. [UniProtKB/Swiss-Prot Function]
Synonyms	DTR; DTS; DTSF; HEGFL
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, GnRH signaling pathway

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