Arp3 (ACTR3) Mouse Monoclonal Antibody [Clone ID: OTI5G4]

Specifications

Product Data
Clone Name	OTI5G4
Applications	IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 2-418 of human ACTR3 (NP_005712) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	47.2 kDa
Gene Name	actin related protein 3
Database Link	NP_005712 Entrez Gene 74117 MouseEntrez Gene 81732 RatEntrez Gene 10096 Human UniProt ID P61158
Background	The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
Synonyms	ARP3
Reference Data
Protein Families	Druggable Genome

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