RNF4 Mouse Monoclonal Antibody [Clone ID: OTI9F10]

Specifications

Product Data
Clone Name	OTI9F10
Applications	WB
Recommend Dilution	WB 1:500~2000
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-190 of human RNF4 (NP_002929) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ring finger protein 4
Database Link	NP_001171938 Entrez Gene 19822 MouseEntrez Gene 29274 RatEntrez Gene 6047 Human UniProt ID P78317
Background	The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Jul 2010]
Synonyms	RES4-26; SLX5; SNURF
Reference Data
Protein Families	Transcription Factors

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