RNF14 Mouse Monoclonal Antibody [Clone ID: OTI3A4]

Specifications

Product Data
Clone Name	OTI3A4
Applications	WB
Recommend Dilution	WB 1:200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-220 of human RNF14 (NP_899647) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	53.7 kDa
Gene Name	ring finger protein 14
Database Link	NP_899647 Entrez Gene 56736 MouseEntrez Gene 619577 RatEntrez Gene 9604 Human UniProt ID Q9UBS8
Background	The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]
Synonyms	ARA54; HFB30; HRIHFB2038; TRIAD2
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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