JIP1 (MAPK8IP1) Mouse Monoclonal Antibody [Clone ID: OTI1D9]

Specifications

Product Data
Clone Name	OTI1D9
Applications	IHC, WB
Recommend Dilution	IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human MAPK8IP1 (NP_005447) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	77.3 kDa
Gene Name	mitogen-activated protein kinase 8 interacting protein 1
Database Link	NP_005447 Entrez Gene 19099 MouseEntrez Gene 116457 RatEntrez Gene 9479 Human UniProt ID Q9UQF2
Background	This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]
Synonyms	IB1; JIP-1; JIP1; PRKM8IP
Reference Data
Protein Families	Druggable Genome
Protein Pathways	MAPK signaling pathway

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