P4HA3 Mouse Monoclonal Antibody [Clone ID: OTI9A2]

Specifications

Product Data
Clone Name	OTI9A2
Applications	IHC
Recommend Dilution	IHC 1:1000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombiant protein of human P4HA3 (NP_878907) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	59.3 kDa
Gene Name	prolyl 4-hydroxylase subunit alpha 3
Database Link	NP_878907 Entrez Gene 320452 MouseEntrez Gene 361612 RatEntrez Gene 283208 Human UniProt ID Q7Z4N8
Background	This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Synonyms	2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha III subunit; alpha polypeptide III; C-P4H alpha III; collagen prolyl 4-hydroxylase alpha(III); procollagen-proline; prolyl 4-hydroxylase
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arginine and proline metabolism, Metabolic pathways

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