CD34 Mouse Monoclonal Antibody [Clone ID: OTI12F2]

Specifications

Product Data
Clone Name	OTI12F2
Applications	IHC, WB
Recommend Dilution	IHC 1:150
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 312-385 of human CD34(NP_001020280) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CD34 molecule
Database Link	NP_001020280 Entrez Gene 947 Human UniProt ID P28906
Background	The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Synonyms	CD34 antigen; CD34 molecule; hematopoietic progenitor cell antigen CD34; OTTHUMP00000034733; OTTHUMP00000034734
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Hematopoietic cell lineage

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