HIF3 alpha (HIF3A) Mouse Monoclonal Antibody [Clone ID: OTI5B7]

Specifications

Product Data
Clone Name	OTI5B7
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 355-669 of human HIF3A (NP_690008) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	72.3 kDa
Gene Name	hypoxia inducible factor 3 subunit alpha
Database Link	NP_690008 Entrez Gene 64344 Human UniProt ID Q9Y2N7
Background	The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Synonyms	bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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