SPARC Mouse Monoclonal Antibody [Clone ID: OTI1D6]

Specifications

Product Data
Clone Name	OTI1D6
Applications	WB
Recommend Dilution	WB 1:4000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human SPARC(NP_003109) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34.5 kDa
Gene Name	secreted protein acidic and cysteine rich
Database Link	NP_003109 Entrez Gene 20692 MouseEntrez Gene 24791 RatEntrez Gene 6678 Human UniProt ID P09486
Background	This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
Synonyms	BM-40; OI17; ON
Reference Data
Protein Families	Druggable Genome

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