MRPS34 Mouse Monoclonal Antibody [Clone ID: OTI4E8]

Specifications

Product Data
Clone Name	OTI4E8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500~1000, IHC 1:150, IF 1:100
Reactivity	Human, Dog, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	25.5 kDa
Gene Name	mitochondrial ribosomal protein S34
Database Link	NP_076425 Entrez Gene 79044 MouseEntrez Gene 100856578 DogEntrez Gene 65993 Human UniProt ID P82930
Background	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Synonyms	MRP-S12; MRP-S34; MRPS12
Reference Data

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