NONO Mouse Monoclonal Antibody [Clone ID: OTI3F8]

Specifications

Product Data
Clone Name	OTI3F8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:200~1000, IHC 1:150, IF 1:100
Reactivity	Human, Dog, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	54.1 kDa
Gene Name	non-POU domain containing octamer binding
Database Link	NP_031389 Entrez Gene 53610 MouseEntrez Gene 317259 RatEntrez Gene 612773 DogEntrez Gene 697263 MonkeyEntrez Gene 4841 Human UniProt ID Q15233
Background	This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
Synonyms	MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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