PPM1G Mouse Monoclonal Antibody [Clone ID: OTI1C12]

Specifications

Product Data
Clone Name	OTI1C12
Applications	IF, WB
Recommend Dilution	WB 1:500~2000, IF 1:100
Reactivity	Human, Dog, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	59.1 kDa
Gene Name	protein phosphatase, Mg2+/Mn2+ dependent 1G
Database Link	NP_817092 Entrez Gene 475703 DogEntrez Gene 5496 Human UniProt ID O15355
Background	The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Synonyms	PP2CG; PP2CGAMMA; PPP2CG
Reference Data
Protein Families	Druggable Genome, Phosphatase

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