ERK5 (MAPK7) Mouse Monoclonal Antibody [Clone ID: OTI5C7]

Specifications

Product Data
Clone Name	OTI5C7
Applications	IF, WB
Recommend Dilution	WB 1:2000, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	88.2 kDa
Gene Name	mitogen-activated protein kinase 7
Database Link	NP_002740 Entrez Gene 23939 MouseEntrez Gene 114509 RatEntrez Gene 5598 Human UniProt ID Q13164
Background	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq]
Synonyms	BMK1; ERK4; ERK5; PRKM7
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway

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