ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI2D5]

Specifications

Product Data
Clone Name	OTI2D5
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	72.3 kDa
Gene Name	Rho GTPase activating protein 25
Database Link	NP_055697 Entrez Gene 232201 MouseEntrez Gene 500246 RatEntrez Gene 9938 Human UniProt ID P42331
Background	ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM]
Synonyms	HEL-S-308; KAIA0053
Reference Data

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