BHMT Mouse Monoclonal Antibody [Clone ID: OTI2F6]

Specifications

Product Data
Clone Name	OTI2F6
Applications	IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:50
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	44.8 kDa
Gene Name	betaine--homocysteine S-methyltransferase
Database Link	NP_001704 Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 635 Human UniProt ID Q93088
Background	This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Synonyms	BHMT1; HEL-S-61p
Reference Data
Protein Pathways	Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways

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