IBRDC2 (RNF144B) Mouse Monoclonal Antibody [Clone ID: OTI10A12]

Specifications

Product Data
Clone Name	OTI10A12
Applications	WB
Recommend Dilution	WB 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	33.7 kDa
Gene Name	ring finger protein 144B
Database Link	NP_877434 Entrez Gene 218215 MouseEntrez Gene 255488 Human UniProt ID Q7Z419
Background	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.
Synonyms	bA528A10.3; IBRDC2; p53RFP; PIR2
Reference Data
Protein Families	Transmembrane

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