BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]

Specifications

Product Data
Clone Name	OTI11C9
Applications	IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46.4 kDa
Gene Name	branched chain keto acid dehydrogenase kinase
Database Link	NP_005872 Entrez Gene 12041 MouseEntrez Gene 29603 RatEntrez Gene 10295 Human UniProt ID O14874
Background	Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .
Synonyms	BCKDKD; BDK
Reference Data
Protein Families	Druggable Genome, Protein Kinase

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