DHFR Mouse Monoclonal Antibody [Clone ID: OTI6G7]

Specifications

Product Data
Clone Name	OTI6G7
Applications	IF, IHC, WB
Recommend Dilution	WB 1:2000 IHC 1:50 IF 1:100
Reactivity	Human, Monkey, Dog, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human DHFR expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	21.5 kDa
Gene Name	dihydrofolate reductase
Database Link	NP_000782 Entrez Gene 13361 MouseEntrez Gene 24312 RatEntrez Gene 479165 DogEntrez Gene 711268 MonkeyEntrez Gene 1719 Human UniProt ID P00374
Background	Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Synonyms	DHFRP1; DYR
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Folate biosynthesis, Metabolic pathways, One carbon pool by folate

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