Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: OTI1H10]

Specifications

Product Data
Clone Name	OTI1H10
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500~1000, IHC 1:50, IF 1:100
Reactivity	Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	47.9 kDa
Gene Name	keratin 18
Database Link	NP_954657 Entrez Gene 16668 MouseEntrez Gene 294853 RatEntrez Gene 3875 Human UniProt ID P05783
Background	Keratin 18 together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Synonyms	CK-18; CYK18; K18
Reference Data
Protein Pathways	Pathogenic Escherichia coli infection

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